Why end capping

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Difference between the normal and end capped columns. Dear all, what the difference between normal reversed phase and endcapped columns regrads, ashwin. Re: Difference between the normal and end capped columns.

The most common reversed-phase columns are manufactured by reacting silica with a silane containing an alkane sidechain. If the alkane is a C group, then it's so large that steric hindrance between adjacent C groups prevents the reaction with some of the silanol groups that lie between them on the surface of the silica.

Such materials are termed nonendcapped. Many of the unreacted silanols can be capped by a second reaction with a silane containing a small alkyl group; usually, C-1 i.

These products are called endcapped. Endcapped materials have a higher carbon load on the surface and avoid most of the reactions with silanols that can lead to skewed peaks.